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Quantitative unspun-urine microscopy as a quick, reliable examination for bacteriuria.
- Source :
-
Scandinavian journal of clinical and laboratory investigation [Scand J Clin Lab Invest] 2005; Vol. 65 (2), pp. 125-32. - Publication Year :
- 2005
-
Abstract
- The diagnosis and treatment of urinary infection are often delayed, causing renal damage, largely because of the unavailability of quick, accurate, diagnostic examinations. Three hundred and twenty-five urine samples from 130 patients were examined for significant bacteriuria using the standard culture method. The urine samples were also examined using the Gram-stain method and quantitative unspun-urine microscopy. When particles could not be distinguished definitely as bacilli by quantitative microscopy, the unspun urine was examined on a slide glass using oil-immersion microscopy at x 1000 magnification. Significant bacteriuria in 37 urine samples was detected by bacterial culture. Using quantitative microscopy, rods were found in 30, cocci in a chain in 3, and indefinite particles in 44 samples. In the 44 indefinite samples, oil-immersion microscopy was able to distinguish rods in one, cocci in a chain in one, cocci in a cluster in two, and negative in 40, which were confirmed by culture as rods, streptococci, staphylococci, and negative, respectively. The quantitative microscopy method was similarly reliable (94.6% sensitivity, 99.3% specificity) for diagnosis of significant bacteriuria when compared with the Gram-stain method (89.2% sensitivity, 98.6% specificity). Quantitative unspun-urine microscopy, confirmed by oil-immersion, is a quick, reliable method for diagnosis of significant bacteriuria, and is considered to be useful for early diagnosis of urinary infection.
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Child
Child, Preschool
Female
Gentian Violet
Humans
Infant
Male
Middle Aged
Phenazines
Reproducibility of Results
Sensitivity and Specificity
Staphylococcus classification
Staphylococcus cytology
Staphylococcus isolation & purification
Streptococcus classification
Streptococcus cytology
Streptococcus isolation & purification
Urinalysis instrumentation
Urinary Tract Infections diagnosis
Urinary Tract Infections microbiology
Bacteriuria diagnosis
Bacteriuria urine
Microbiological Techniques
Microscopy
Urinalysis methods
Subjects
Details
- Language :
- English
- ISSN :
- 0036-5513
- Volume :
- 65
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Scandinavian journal of clinical and laboratory investigation
- Publication Type :
- Academic Journal
- Accession number :
- 16025835
- Full Text :
- https://doi.org/10.1080/00365510510013514