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Inherent potential for production of tumor necrosis factor-alpha by human intestinal macrophages.
- Source :
-
International journal of colorectal disease [Int J Colorectal Dis] 2006 May; Vol. 21 (4), pp. 339-47. Date of Electronic Publication: 2005 Aug 10. - Publication Year :
- 2006
-
Abstract
- Background and Aims: Tumor necrosis factor (TNF) production by the macrophages in intestines appears to play a critical role in the pathogenesis of Crohn's disease (CD). However, it is reported that resident intestinal macrophages (both colonic and small-bowel) do not produce TNF after lipopolysaccharide (LPS) stimulation. It has not yet been proven whether or not intestinal macrophages have an inherent potential to produce TNF. The purpose of this study is to answer this question.<br />Materials and Methods: Colonic macrophages were isolated from lamina propria of human large intestine and stimulated with a variety of substances: LPS, a lipid A derivative (ONO-4007), killed Streptococcus bacterial body (OK-432), phorbol 12-myristate 13-acetate, and lectins (pokeweed mitogen and Sarcophaga lectin).<br />Results: Colonic macrophages were phenotypically negative for CD14 and positive for CD68 and produced very little TNF in response to LPS, as reported previously. Of the substances tested, only Sarcophaga lectin, which is a defense protein of fleshflies (Sarcophaga peregrina), induced TNF production by the intestinal macrophages. In addition, when the colonic macrophages were cultured on immunoglobulin-A-coated dishes, their characteristic response to LPS was altered, and they produced TNF at a level 6.6 times higher than when on collagen-coated dishes.<br />Conclusion: Colonic macrophages have an inherent ability to produce TNF. Activation of colonic macrophages by unknown substances may contribute to the induction of TNF production, which causes the intestinal inflammation of CD.
- Subjects :
- Antigens, CD
Antigens, Differentiation, Myelomonocytic
Antineoplastic Agents pharmacology
Carcinogens pharmacology
Cells, Cultured
Collagen
Culture Techniques instrumentation
Humans
Immunoglobulin A
Insect Proteins pharmacology
Lectins, C-Type
Lipopolysaccharides pharmacology
Mitogens pharmacology
Picibanil pharmacology
Pokeweed Mitogens pharmacology
Tetradecanoylphorbol Acetate
Colon metabolism
Macrophage Activation
Macrophages metabolism
Tumor Necrosis Factor-alpha biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0179-1958
- Volume :
- 21
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- International journal of colorectal disease
- Publication Type :
- Academic Journal
- Accession number :
- 16091914
- Full Text :
- https://doi.org/10.1007/s00384-005-0021-5