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Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2005 Aug 21; Vol. 11 (31), pp. 4788-93. - Publication Year :
- 2005
-
Abstract
- Aim: To study the effect of IL-10 on the expression of growth factors--transforming growth factor-beta1 (TGF-beta1), epidermal growth factor (EGF), hepatocyte growth factor (HGF) and platelet-derived growth factor (PDGF) of hepatic stellate cells (HSCs) of hepatic fibrosis rat and the anti-fibrogenic role of exogenous IL-10.<br />Methods: Hepatic fibrosis was induced by CCl(4) administration intra-peritoneally. Sixty clean male Sprague-Dawley (SD) rats were randomly divided into three groups: normal control group (GN, 8 rats), hepatic fibrosis model group (GC, 28 rats) and IL-10 treated group (GI, 24 rats). At the beginning of the 7th and 11th wk, rats in each group were routinely perfused with pronase E and type IV collagenase through a portal vein catheter and the suspension obtained from the liver was spun by centrifugation with 11% Nycodenz density gradient to isolate HSCs. Histological examination was used to determine the degree of hepatic fibrosis. RT-PCR was employed to analyze mRNA expression from freshly isolated cells. Immunocytochemistry was performed to detect protein expression in primary cultured HSCs.<br />Results: Rat hepatic fibrosis was developed with the increase of injection frequency of CCl(4), and HSCs were successfully isolated. At the 7th and 11th wk, TGF-beta1, EGF, and HGF mRNA in GC increased obviously compared with GN (P = 0.001/0.042, 0.001/0.001, 0.001/0.001) and GI (P = 0.001/0.007, 0.002/0.001, 0.001/0.001). For TGF-beta1, no difference was observed between GI and GN. For EGF, mRNA level in GI increased compared with GN during the 7th wk (P = 0.005) and 11th wk (P = 0.049). For HGF, mRNA level in GI decreased compared with GN at the 7th wk (P = 0.001) and 11th wk (P = 0.021). Between these two time points, TGF-beta1 expression at the 7th wk was higher than that of the 11th wk (P = 0.049), but for EGF, the former was lower than the latter (P = 0.022). As for PDGF mRNA, there was no significant difference between these groups, but difference seemed to exist in protein levels. Results by immunocytochemistry of TGF-beta1 and EGF were paralleled with the above findings.<br />Conclusion: The expression of TGF-beta1, EGF and HGF increased in HSC of hepatic fibrosis rat and decreased after treatment with IL-10. IL-10 plays an anti-fibrogenic role by suppressing growth factors expression.
- Subjects :
- Animals
Base Sequence
Carbon Tetrachloride Poisoning metabolism
DNA Primers
Gene Expression Regulation drug effects
Liver Diseases pathology
Male
RNA, Messenger genetics
Rats
Rats, Sprague-Dawley
Transforming Growth Factor beta1
Carbon Tetrachloride Poisoning pathology
Epidermal Growth Factor genetics
Hepatocyte Growth Factor genetics
Interleukin-10 pharmacology
Liver Diseases metabolism
Transforming Growth Factor beta genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1007-9327
- Volume :
- 11
- Issue :
- 31
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 16097045
- Full Text :
- https://doi.org/10.3748/wjg.v11.i31.4788