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Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders.
- Source :
-
Asian journal of andrology [Asian J Androl] 2005 Sep; Vol. 7 (3), pp. 303-9. - Publication Year :
- 2005
-
Abstract
- Aim: To evaluate two extenders and two cryoprotectant agents (CPA) for alpaca semen cryopreservation.<br />Methods: Semen samples were obtained from four adult alpacas (Lama pacos) and frozen using extender I (TRIS, citrate, egg yolk and glucose) or extender II (skim milk, egg yolk and fructose), each containing either glycerol (G) or ethylene glycol (EG) as CPA. Consequently, four groups were formed: 1) extender I-G; 2) extender I-EG; 3) extender II-G; and 4) extender II-EG. Semen was diluted in a two-step process: for cooling to 5 degrees (extenders without CPA), and for freezing (extenders with CPA). Viability and acrosome integrity were assessed using trypan blue and Giemsa stains.<br />Results: When compared, the motility after thawing was higher (P >0.05) in groups II-EG (20.0% +/- 6.7%) and II-G (15.3 % +/- 4.1% ) than that in groups I-G (4.0 % +/- 1.1%) and I-EG (1.0 % +/- 1.4%). Viable spermatozoa with intact acrosomes in groups II-EG (18.7 % +/- 2.9%) and II-G (12.7 % +/- 5.9%) were higher than that in groups I-G (5.7% +/- 1.5%) and I-EG (4.0% +/- 1.0%).<br />Conclusion: The skim milk- and egg yolk-based extenders containing ethylene glycol or glycerol to freeze alpaca semen seems to promote the survival of more sperm cells with intact acrosomes than the other extenders.
Details
- Language :
- English
- ISSN :
- 1008-682X
- Volume :
- 7
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Asian journal of andrology
- Publication Type :
- Academic Journal
- Accession number :
- 16110359
- Full Text :
- https://doi.org/10.1111/j.1745-7262.2005.00021.x