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Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds.
- Source :
-
Antiviral research [Antiviral Res] 2005 Oct; Vol. 68 (1), pp. 36-42. - Publication Year :
- 2005
-
Abstract
- The 3C-like protease (3CLpro) of SARS-coronavirus mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins, becoming an important target for the drug development. In this study, Isatis indigotica root extract, five major compounds of I. indigotica root, and seven plant-derived phenolic compounds were tested for anti-SARS-CoV 3CLpro effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CLpro demonstrated that IC50 values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC50: 217 microM) was more efficient in blocking the cleavage processing of the 3CLpro than indigo (IC50: 752 microM) and beta-sitosterol (IC50: 1210 microM) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin dose-dependently inhibited cleavage activity of the 3CLpro, in which the IC50 was 366 microM for aloe emodin and 8.3 microM for hesperetin in the cell-based assay.
- Subjects :
- Animals
Anthraquinones
Chlorocebus aethiops
Coloring Agents chemistry
Coloring Agents pharmacology
Coronavirus 3C Proteases
Cysteine Endopeptidases
Emodin chemistry
Emodin pharmacology
Endopeptidases metabolism
Enzyme Inhibitors chemistry
Enzyme Inhibitors pharmacology
Glucosinolates chemistry
Glucosinolates pharmacology
Hesperidin chemistry
Hesperidin pharmacology
Indigo Carmine
Indoles chemistry
Indoles pharmacology
Plant Extracts pharmacology
Plant Roots chemistry
Vero Cells metabolism
Viral Proteins metabolism
Endopeptidases drug effects
Isatis chemistry
Phenols pharmacology
Viral Proteins drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0166-3542
- Volume :
- 68
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Antiviral research
- Publication Type :
- Academic Journal
- Accession number :
- 16115693
- Full Text :
- https://doi.org/10.1016/j.antiviral.2005.07.002