Back to Search
Start Over
Leukemia inhibitory factor triggers activation of signal transducer and activator of transcription 3, proliferation, invasiveness, and altered protease expression in choriocarcinoma cells.
Leukemia inhibitory factor triggers activation of signal transducer and activator of transcription 3, proliferation, invasiveness, and altered protease expression in choriocarcinoma cells.
- Source :
-
The international journal of biochemistry & cell biology [Int J Biochem Cell Biol] 2005 Nov; Vol. 37 (11), pp. 2284-96. Date of Electronic Publication: 2005 Mar 17. - Publication Year :
- 2005
-
Abstract
- Extravillous trophoblast cells resemble cancer cells with regard to their intrinsic invasiveness. They invade decidual tissue, but, unlike tumor cells, shut down their invasive properties, when they become inappropriate. Stimuli involved in the modulation of invasion, as well as their underlying signaling mechanisms require further clarification. We were especially interested in discovering signals capable of stimulating invasion in otherwise low-invasive cells involved in reproduction. Using the choriocarcinoma cell line Jeg-3 as a model, we have addressed the potential role of cytokine/growth factor-driven activation of signal transducer and activator of transcription 3 (STAT3) in this process. Jeg-3 cells were treated with various factors known to induce trophoblast proliferation, differentiation, migration, or invasiveness (insulin-like-growth-factor-II (IGF-II), hepatocyte growth factor (HGF), interleukin-6 (IL-6), and leukemia inhibitory factor (LIF)). Only LIF elicited strong tyrosine phosphorylation and specific DNA-binding activity of STAT3. It induced a significant acceleration of cell proliferation and promoted the capability of Jeg-3 cells to invade into an artificial extracellular matrix. Moreover, LIF influenced the expression pattern of proteases and protease inhibitors with potential relevance for invasiveness (downregulation of mRNA for tissue inhibitor of metalloproteinase 1 (TIMP-1) and upregulation of mRNA for caspase-4). In conjunction with earlier work, in which we found that STAT3 DNA-binding activity was increased in invasive cells (choriocarcinoma, first trimester trophoblasts) and absent in non-invasive cells (term trophoblasts), these findings suggest a connection between LIF-driven STAT3 activity and invasiveness of choriocarcinoma and trophoblast cells.
- Subjects :
- Caspases metabolism
Caspases, Initiator
Cell Line, Tumor
Female
Gene Expression Profiling
Humans
Leukemia Inhibitory Factor
Neoplasm Invasiveness
Oligonucleotide Array Sequence Analysis
Peptide Hydrolases genetics
Phosphorylation
Pregnancy
Tissue Inhibitor of Metalloproteinase-1 metabolism
Tyrosine metabolism
Cell Proliferation
Choriocarcinoma metabolism
Choriocarcinoma pathology
Interleukin-6 metabolism
Peptide Hydrolases metabolism
STAT3 Transcription Factor metabolism
Signal Transduction physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1357-2725
- Volume :
- 37
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The international journal of biochemistry & cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 16125646
- Full Text :
- https://doi.org/10.1016/j.biocel.2005.02.025