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LC-DAD-APCI-MS-based screening and analysis of the absorption and metabolite components in plasma from a rabbit administered an oral solution of danggui.
- Source :
-
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2005 Sep; Vol. 383 (2), pp. 247-54. Date of Electronic Publication: 2005 Oct 12. - Publication Year :
- 2005
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Abstract
- A valid chromatographic fingerprint method using liquid chromatography-diode array detection-atmospheric pressure chemical ionization mass spectrometry in negative mode (LC-DAD-APCI-MS) is proposed for studying the absorption and metabolites of a traditional Chinese medicine (TCM) Angelica sinensis (danggui) in rabbit plasma, after the rabbit is administered with danggui oral solution (DOS). More than thirty-two common components were detected in both DOS and rabbit plasma, which shows that the components in the DOS were absorbed into the body of the rabbit. Of these, senkyunolide I, senkyunolide H, Z-6,7-epoxyligustilide, 3-butylidene-7-hydroxyphthalide, Z-ligustilide, Z-butylidenephthalide, Diels-Alder dimers of ligustilide, linolenic acid, linoleic acid and falcarindiol were tentatively identified from their MS, UV spectra and retention behavior by comparing the results with the published literature. At least ten components were found in rabbit plasma but not in DOS, indicating that these components must be metabolites of some of the components in the original extract. The results prove that the proposed method can be used to rapidly analyze multiple constituents in TCMs, and to screen for bioactive compounds by comparing and contrasting the chromatographic fingerprints of DOS and plasma samples.
- Subjects :
- 4-Butyrolactone analogs & derivatives
4-Butyrolactone blood
Absorption
Administration, Oral
Aldehydes blood
Animals
Benzofurans blood
Diynes
Drugs, Chinese Herbal administration & dosage
Fatty Alcohols blood
Linoleic Acid blood
Mass Spectrometry methods
Phthalic Anhydrides blood
Plant Extracts administration & dosage
Plant Extracts chemistry
Rabbits
alpha-Linolenic Acid blood
Angelica sinensis chemistry
Drugs, Chinese Herbal chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1618-2642
- Volume :
- 383
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Analytical and bioanalytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 16132135
- Full Text :
- https://doi.org/10.1007/s00216-005-0008-7