[Depression of mPges expression by troglitazone in human renal glomerulus induced by high glucose and LPS].

Objective: To determine the influence of troglitanzone on the expression of mPGEs induced by high glucose or LPS in human glomeruli cultured in vitro.
Methods: Human glomeruli were isolated and incubated by LPS or high glucose absence or presence with different concentrations of troglitazone. The concentration of mPGEs in the supernatant was measured by an enzyme-linked immunosorbent assay (ELISA).
Results: LPS (10 microg/ml) and 5% glucose could improve the concentration of mPGEs significantly in the supernatant by cultured human glomeruli, and troglitazone (10 - 20 nmol/L) could block its increase (P < 0.05).
Conclusion: High glucose and LPS can improve mPGEs expression in cultured human glomeruli, and troglitazone can block this effects. These data suggest that PPAR-gamma agonist troglitanzone may be relevant to the treatment of diabetic nephropathy and inflammation in glomerluli.