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Differential aromatase (CYP19) expression in human arteries from normal and neoplasic uterus: an immunohistochemical and in situ hybridization study.
- Source :
-
Frontiers in bioscience : a journal and virtual library [Front Biosci] 2006 Jan 01; Vol. 11, pp. 389-93. Date of Electronic Publication: 2006 Jan 01. - Publication Year :
- 2006
-
Abstract
- Aromatase CYP19 catalyzes the synthesis of estrogen from androgens in a tissue-specific manner. This enzyme is present in several tissues, including gonads, brain and fatty tissue. More recently, its presence has been described in vessels. Here, we describe the expression of aromatase in human uterine artery and compare its expression with that found in arteries of estrogen-dependent uterine leiomyomata from women. To do this, we employed immunohystochemical and in situ hybridization techniques. We used, a polyclonal antibody raised against the carboxyl terminus of aromatase (ARO) and RNAm probes, of the exon 1 of ARO. We found an increased immunoreactivity of ARO in uterine arteries of patients with leiomyoma as compared with control group. Probe showing positive signal in skin fibroblasts (1b), showed positive hybridization signal in normal artery, while probes with positive signal in placenta (1a), ovary (1c) and testis (1d) were over-expressed in arteries of leiomyomas.
- Subjects :
- Aromatase chemistry
Aromatase metabolism
Estrogens metabolism
Exons
Female
Humans
Immunohistochemistry
In Situ Hybridization
Male
Models, Statistical
Neovascularization, Pathologic
Oligonucleotide Probes chemistry
Ovary metabolism
Placenta metabolism
Protein Structure, Tertiary
Testis metabolism
Tissue Distribution
Uterine Neoplasms blood supply
Uterine Neoplasms pathology
Uterus blood supply
Uterus pathology
Aromatase biosynthesis
Arteries metabolism
Leiomyoma metabolism
Uterine Neoplasms enzymology
Uterus enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1093-9946
- Volume :
- 11
- Database :
- MEDLINE
- Journal :
- Frontiers in bioscience : a journal and virtual library
- Publication Type :
- Academic Journal
- Accession number :
- 16146740
- Full Text :
- https://doi.org/10.2741/1806