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Evaluation of a capillary immunoassay system for detection of Salmonella typhimurium in poultry products.
- Source :
-
Journal of food protection [J Food Prot] 2005 Sep; Vol. 68 (9), pp. 1799-803. - Publication Year :
- 2005
-
Abstract
- A capillary immunoassay system was constructed and optimized for detection of Salmonella Typhimurium. The system consisted of a capillary bioseparator-bioreactor and a flow-injection electrochemical detector. Three methods were compared for immobilizing antibodies on the inner surface of silica capillary columns; these methods were based on the use of a homobifunctional cross-linker glutaraldehyde, a heterobifunctional cross-linker N-succinimidyl-4-maleimidobutyrate, and biotin-streptavidin chemistry, respectively. The glutaraldehyde method gave the best reproducibility with a relative standard deviation of 1 to 6% for detection of Salmonella Typhimurium. The optimized immunoassay system could detect Salmonella Typhimurium in chicken breast and ground turkey meats with a detection limit of 2.4 x 10(3) and 2.4 x 10(4) CFU/ml, respectively. The total detection time was less than 2.5 h without any preenrichment. When stored at 4 degrees C, the immunocolumns could retain their activities for at least 3 months.
- Subjects :
- Bioreactors microbiology
Colony Count, Microbial
Food Microbiology
Salmonella typhimurium isolation & purification
Sensitivity and Specificity
Time Factors
Antibodies, Bacterial analysis
Food Contamination analysis
Immunoenzyme Techniques methods
Poultry Products microbiology
Salmonella typhimurium immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0362-028X
- Volume :
- 68
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of food protection
- Publication Type :
- Academic Journal
- Accession number :
- 16161676
- Full Text :
- https://doi.org/10.4315/0362-028x-68.9.1799