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Evidences that beta1 integrin and Rac1 are involved in the overriding effect of laminin on myelin-associated glycoprotein inhibitory activity on neuronal cells.
- Source :
-
Molecular and cellular neurosciences [Mol Cell Neurosci] 2005 Nov; Vol. 30 (3), pp. 418-28. Date of Electronic Publication: 2005 Sep 15. - Publication Year :
- 2005
-
Abstract
- During neurite elongation, migrating growth cones encounter both permissive and inhibitory substrates, such as laminin and MAG (myelin-associated glycoprotein), respectively. Here, we demonstrated on two neuronal cell lines (PC12 and N1E-115), that laminin and collagen hampered, in a dose-dependent manner, MAG inhibitory activity on several integrin functions, i.e., neurite growth, cell adhesion and cell spreading. Using a function blocking antibody, in PC12 cells, we showed that alpha1beta1 integrin is required in these phenomena. In parallel, we observed that MAG perturbs actin dynamics and lamellipodia formation during early steps of cell spreading. This seemed to be independent of RhoA activation, but dependent of Rac-1 inhibition by MAG. Laminin overrode MAG activity on actin and prevented MAG inhibition NGF-induced Rac1 activation. In conclusion, we evidenced antagonistic signaling between MAG receptors and beta1 integrins, in which Rac-1 may have a central function.
- Subjects :
- Animals
Antibodies pharmacology
Cattle
Cell Differentiation drug effects
Cell Differentiation physiology
Cell Line
Cell Line, Tumor
Cell Movement drug effects
Cell Movement physiology
Central Nervous System cytology
Central Nervous System embryology
Central Nervous System metabolism
Collagen metabolism
Collagen pharmacology
Dose-Response Relationship, Drug
Feedback, Physiological drug effects
Feedback, Physiological physiology
Growth Cones drug effects
Growth Cones metabolism
Growth Cones ultrastructure
Humans
Integrin alpha1beta1 metabolism
Laminin pharmacology
Mice
PC12 Cells
Rats
Receptors, Cell Surface drug effects
Receptors, Cell Surface metabolism
Signal Transduction drug effects
Signal Transduction physiology
Integrin beta1 metabolism
Laminin metabolism
Myelin-Associated Glycoprotein metabolism
Neurons metabolism
rac1 GTP-Binding Protein metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1044-7431
- Volume :
- 30
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molecular and cellular neurosciences
- Publication Type :
- Academic Journal
- Accession number :
- 16168664
- Full Text :
- https://doi.org/10.1016/j.mcn.2005.08.006