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Rational control of enantioselectivity of lipase by site-directed mutagenesis based on the mechanism.

Authors :
Ema T
Fujii T
Ozaki M
Korenaga T
Sakai T
Source :
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2005 Oct 07 (37), pp. 4650-1. Date of Electronic Publication: 2005 Aug 12.
Publication Year :
2005

Abstract

The enantioselectivity of a Burkholderia cepacia lipase toward secondary alcohols could be both increased and decreased rationally by introducing only a single mutation on the basis of the mechanism proposed previously.

Details

Language :
English
ISSN :
1359-7345
Issue :
37
Database :
MEDLINE
Journal :
Chemical communications (Cambridge, England)
Publication Type :
Academic Journal
Accession number :
16175280
Full Text :
https://doi.org/10.1039/b508244g