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Comparative efficacies of quinupristin-dalfopristin, linezolid, vancomycin, and ciprofloxacin in treatment, using the antibiotic-lock technique, of experimental catheter-related infection due to Staphylococcus aureus.
- Source :
-
Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2005 Oct; Vol. 49 (10), pp. 4042-5. - Publication Year :
- 2005
-
Abstract
- We performed in vitro studies to elucidate the bactericidal activity of the antibiotics in an adherent-cell biofilm model. Efficacy studies were performed in a staphylococcal central venous catheter (CVC) infection rat model. Silastic catheters were implanted into the superior cava. Via the CVC the rats were challenged with 1.0 x 10(6) CFU of a live Staphylococcus aureus strain. Twenty-four hours later, the antibiotic-lock technique was started. All animals were randomized to receive daily isotonic sodium chloride solution, quinupristin-dalfopristin (Q/D), linezolid, vancomycin, or ciprofloxacin at the minimal bactericidal concentration (MBC) and at 1,024 microg/ml in a volume of 0.1 ml that filled the CVC. The main outcome measures were MICs and MBCs for both planktonic and adherent cells, quantitative culture of the catheters and surrounding venous tissues, and quantitative peripheral blood cultures. The killing activities of all antibiotics against the adherent bacteria were at least fourfold lower than those against freely growing cells, with the exception of Q/D, which showed comparable activities against both adherent and planktonic organisms. Overall, Q/D at 1,024 microg/ml produced the greatest reduction in the number of cells recovered from the catheters, while at the same concentration, Q/D and vancomycin demonstrated higher activities than ciprofloxacin or linezolid in reducing the number of organisms recovered from the blood cultures. This study points out that treatment outcome of device-related infections cannot be predicted by the results of a standard susceptibility test such as the MIC. Our findings suggest that the clinically used antibiotics cannot eradicate the CVC infection through the antibiotic-lock technique, even at a concentration of 1,024 microg/ml.
- Subjects :
- Animals
Biofilms drug effects
Biofilms growth & development
Catheters, Indwelling microbiology
Disease Models, Animal
Drug Therapy, Combination
Linezolid
Male
Plankton drug effects
Plankton microbiology
Rats
Rats, Wistar
Staphylococcal Infections etiology
Staphylococcal Infections microbiology
Staphylococcus aureus genetics
Staphylococcus aureus growth & development
Treatment Outcome
Acetamides pharmacology
Anti-Bacterial Agents pharmacology
Anti-Infective Agents pharmacology
Ciprofloxacin pharmacology
Oxazolidinones pharmacology
Staphylococcal Infections drug therapy
Staphylococcus aureus drug effects
Vancomycin pharmacology
Virginiamycin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0066-4804
- Volume :
- 49
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Antimicrobial agents and chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 16189078
- Full Text :
- https://doi.org/10.1128/AAC.49.10.4042-4045.2005