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Effects of simvastatin on the expression of intercellular adhesion molecule-1 mRNA in neonatal brain with hypoxic-ischemic damage.
- Source :
-
Journal of nanoscience and nanotechnology [J Nanosci Nanotechnol] 2005 Aug; Vol. 5 (8), pp. 1261-5. - Publication Year :
- 2005
-
Abstract
- The goal of this paper is to report on the study of the mechanism of neural damage in neonatal cerebral cortex and hippocampus when hypoxic-ischemic brain damage (HIBD) occurs and the neural protective effects of simvastatin. One hundred forty-four 7-day-old SD rats of were randomly divided into four groups, i.e., sham operation group (sham group), normal saline control group (HIBD group), citicoline control group (CDPC group), and simvastatin-treated group. Each group was divided into six time points (0 h, 12 h, 24 h, 48 h, 72 h, and 7 days after HIBD or sham operation), and each time point included six rats. The expression of intercellular adhesion molecule-1 (ICAM-1) mRNA was determined semiquantitively by reverse transcription polymerase chain reaction (RT-PCR) in the cortex and hippocampus of neonatal rats at different times to determine the differences. The expression of ICAM-1 mRNA rose obviously 12 h after HIBD in the ischemic hemisphere, peaked at 24 h, and then decreased gradually. There was a remarkably lower expression in the CDPC group and simvastatin group compared with the HIBD group at 12 h, 24 h, 48 h, and 72 h. At 7 days, there was no significant difference between the simvastatin group and the sham group (P > 0.05), and at 72 h, simvastatin down-regulated the expression of ICAM-1 mRNA in cortex significantly more than did citicoline (P < 0.05). Both simvastatin and CDPC can relieve the damages of the brain in rats with HIBD. The protective mechanisms of simvastatin and citicoline are associated with their regulative effect on ICAM-1, and simvastatin is superior to citicoline.
- Subjects :
- Animals
Animals, Newborn
Base Sequence
Brain metabolism
Cerebral Cortex drug effects
Cerebral Cortex metabolism
Cytidine Diphosphate Choline pharmacology
DNA, Complementary genetics
Female
Gene Expression drug effects
Hippocampus drug effects
Hippocampus metabolism
Hydroxymethylglutaryl-CoA Reductase Inhibitors pharmacology
Male
Nanotechnology
RNA, Messenger genetics
RNA, Messenger metabolism
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Hypoxia-Ischemia, Brain drug therapy
Hypoxia-Ischemia, Brain genetics
Intercellular Adhesion Molecule-1 genetics
Neuroprotective Agents pharmacology
Simvastatin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1533-4880
- Volume :
- 5
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of nanoscience and nanotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 16193989
- Full Text :
- https://doi.org/10.1166/jnn.2005.224