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Molecular cloning and tissue distribution of SF-1-related orphan receptors during sexual maturation in female goldfish.
- Source :
-
Biotechnology letters [Biotechnol Lett] 2005 Sep; Vol. 27 (17), pp. 1283-90. - Publication Year :
- 2005
-
Abstract
- The steroidogenic factor (SF)-1 gene is one of a number of orphan nuclear receptors, which is a key transcriptional regulator in vertebrate reproduction. We have isolated the SF-1 homologue cDNA from the goldfish pituitary and designed primers for SF-1 on the basis of the highly conserved regions of various known SF-1 superfamily genes. SF-1 cDNA contained 1,948 nucleotides including an open reading frame predicted to encode a protein of 503 amino acids. The distribution pattern of SF-1 in a variety of tissues during sexual maturation in female goldfish was also examined by RT-PCR. Significant variations in the relative expression of SF-1 were observed in different tissues in immature and mature female goldfish. SF-1 transcript in pituitary was significantly higher than other tissues tested in immature and mature female goldfish. Lower expression of SF-1 was observed in the liver but was not detected in brain and ovary of the immature female goldfish. Presence of SF-1 was the predominant expression in the pituitary and brain of mature female goldfish. Also, in the mature female goldfish, a weak transcript was detected in liver and ovary. Interestingly, RT-PCR analysis revealed that the expression of SF-1 became higher in the brain and weaker in the liver in maturing female goldfish. Thus, SF-1 may be regulated in goldfish brain and/or liver. Thus is also tissue-specific distribution of SF-1 during sexual maturation in female goldfish.
- Subjects :
- Animals
Cloning, Molecular
Female
Goldfish genetics
Goldfish growth & development
Homeodomain Proteins genetics
Liver physiology
Open Reading Frames
Organ Specificity physiology
Pituitary Gland physiology
Receptors, Cytoplasmic and Nuclear genetics
Steroidogenic Factor 1
Transcription Factors genetics
Gene Expression Regulation physiology
Goldfish metabolism
Homeodomain Proteins biosynthesis
Receptors, Cytoplasmic and Nuclear biosynthesis
Sexual Maturation physiology
Transcription Factors biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0141-5492
- Volume :
- 27
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Biotechnology letters
- Publication Type :
- Academic Journal
- Accession number :
- 16215826
- Full Text :
- https://doi.org/10.1007/s10529-005-0220-8