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[Antitumor research on human laryngeal carcinoma with combined application of VP3 gene, IL-18 gene and HN gene].
- Source :
-
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology [Lin Chuang Er Bi Yan Hou Ke Za Zhi] 2005 Aug; Vol. 19 (15), pp. 704-6. - Publication Year :
- 2005
-
Abstract
- Objective: To research the antitumor efficacy on mice model bearing H22 tumor with application of pVVP3IL-18HN and its mechanism of the antitumor effect on Hep-2.<br />Method: pVVP3IL-18HN was introduced into Hep-2 cells by liposome, then cellular morphology was observed through AO/EB stain. To evaluate the mechanism of the antitumor effect on Hep-2 of pVVP3IL-18HN and its effect on tumor immunogenicity, flow cytometer (FCM) was used to detect the variation of mitochondrial trans-membrane potential,reactive oxygen species (ROS) and the MHC-I. C57BL/6 mice were subcutaneously inoculated with 2 X 10(5) H22 tumor cells in the right hindlimb, and 7, 14 and 21 day post-inoculation, pVVP3IL-18HN was administrated intratumorally. The pVVP3, pVIL-18, PVHN and PBS control group were treated as above. Then the tumor suppression and specific cytotoxic T lymphocyte (CTL) activity was detected.<br />Result: After the transfection of pVVP3IL-18HN, tumor cells crenated and stained by AO/EB. In the research pVVP3IL-18HN was found to up-regulate the value of ROS and the expression of MHC-I, and down-regulate the value of mitochondrial trans-membrane potential. The tumor suppression rate of pVVP3IL-18HN was 60.5% and a dramatically increased tumor suppression was revealed in the pVVP3IL-18HN treated mice as compared with control mice treated with PBS (P < 0.05).<br />Conclusion: pVVP3IL-18HN kill the tumor cells by apoptosis and have notable antitumor effect in vivo.
- Subjects :
- Animals
Cancer Vaccines genetics
Cell Line, Tumor
Genetic Therapy
Humans
Mice
Mice, Inbred C57BL
Mice, Nude
Transfection
Vaccines, DNA genetics
Xenograft Model Antitumor Assays
Apoptosis
Cancer Vaccines pharmacology
Interleukin-18 genetics
Laryngeal Neoplasms drug therapy
Vaccines, DNA pharmacology
Subjects
Details
- Language :
- Chinese
- Volume :
- 19
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology
- Publication Type :
- Academic Journal
- Accession number :
- 16248512