Back to Search
Start Over
Presynaptic alpha1 adrenergic receptors differentially regulate synaptic glutamate and GABA release to hypothalamic presympathetic neurons.
- Source :
-
The Journal of pharmacology and experimental therapeutics [J Pharmacol Exp Ther] 2006 Feb; Vol. 316 (2), pp. 733-42. Date of Electronic Publication: 2005 Oct 25. - Publication Year :
- 2006
-
Abstract
- The hypothalamic paraventricular nucleus (PVN) neurons that project to the spinal intermediolateral cell column and brainstem are important for the control of sympathetic outflow. Stimulation of alpha(1) adrenergic receptors in the PVN increases sympathetic outflow, but the cellular mechanisms remain unclear. In this study, we determined the role of alpha(1) adrenergic receptors in the regulation of glutamatergic and GABAergic synaptic inputs to spinally projecting PVN neurons. Whole-cell and cell-attached patch-clamp recordings were performed on retrogradely labeled PVN-spinal neurons in rat brain slices. Bath application of 10 to 100 microM phenylephrine, an alpha(1) adrenergic receptor agonist, significantly increased the frequency of spontaneous excitatory postsynaptic currents in a concentration-dependent manner. This effect was blocked by the alpha (1)adrenergic receptor antagonists prazosin or corynanthine. Phenylephrine also significantly increased the frequency of miniature excitatory postsynaptic currents (mEPSCs) but not the amplitude and decay constant of mEPSCs. Furthermore, activation of alpha(1) adrenergic receptors with phenylephrine or cirazoline significantly decreased the frequency of spontaneous inhibitory postsynaptic currents and miniature inhibitory postsynaptic currents, and this effect also was blocked by corynanthine. In addition, 50 microM phenylephrine significantly increased the firing rate of 13 labeled PVN neurons from 3.16 +/- 0.42 to 5.83 +/- 0.65 Hz. However, phenylephrine failed to increase the firing of most labeled PVN neurons in the presence of GABA(A) and ionotropic glutamate receptor antagonists. Thus, these data suggest that activation of alpha (1)adrenergic receptors increases the excitability of PVN presympathetic neurons primarily through augmentation of glutamatergic tone and attenuation of GABAergic inputs.
- Subjects :
- Adrenergic Antagonists pharmacology
Adrenergic alpha-1 Receptor Antagonists
Animals
Excitatory Postsynaptic Potentials physiology
In Vitro Techniques
Male
Neurons metabolism
Paraventricular Hypothalamic Nucleus physiology
Patch-Clamp Techniques
Rats
Rats, Sprague-Dawley
Synapses metabolism
Glutamates metabolism
Neurons physiology
Paraventricular Hypothalamic Nucleus cytology
Receptors, Adrenergic, alpha-1 physiology
Receptors, Presynaptic physiology
Synapses physiology
gamma-Aminobutyric Acid metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3565
- Volume :
- 316
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of pharmacology and experimental therapeutics
- Publication Type :
- Academic Journal
- Accession number :
- 16249372
- Full Text :
- https://doi.org/10.1124/jpet.105.094797