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[Protective effects of ligustrazine against photoreceptor cell injury induced by N-methyl-N-nitrosourea and its mechanism].
- Source :
-
Yao xue xue bao = Acta pharmaceutica Sinica [Yao Xue Xue Bao] 2005 Aug; Vol. 40 (8), pp. 690-4. - Publication Year :
- 2005
-
Abstract
- Aim: To study the protective effect of ligustrazine against photoreceptor cell injury induced by N-methyl-N-nitrosourea (MNU) in Sprague-Dawley (SD) rats.<br />Methods: Ligustrazine injections of different doses were injected intraperitoneally into 47-day female SD rats once a day and a single intraperitoneal injection of MNU 60 mg x kg(-1) was given to 50-day rats. At different intervals after MNU treatment,the animals were sacrificed. The apoptotic index of photoreceptor cells was calculated by TUNEL labeling at 24 h following MNU treatment; peripheral retinal damage was evaluated based on retinal thickness at the d 7 after MNU treatment, and the expression of c-jun and c-fos genes was detected by RT-PCR technique.<br />Results: Ligustrazine injection could remarkably increase total thickness of peripheral retina and decrease apoptotic index of photoreceptor cells induced by MNU in a dose-dependent manner. Compared with MNU-treated rats, the gene expression of c-jun and c-fos was time-dependently down-regulated in ligustrazine-treated group.<br />Conclusion: Ligustrazine injection partially protects against MNU-induced retinal damage by down-modulating the expression of c-jun and c-fos genes to inhibit apoptosis of photoreceptor cells.
- Subjects :
- Animals
Dose-Response Relationship, Drug
Female
Genes, fos
Genes, jun
Injections, Intraperitoneal
Methylnitrosourea
Photoreceptor Cells, Vertebrate drug effects
Photoreceptor Cells, Vertebrate pathology
Plants, Medicinal chemistry
Protective Agents administration & dosage
Protective Agents pharmacology
Pyrazines administration & dosage
Pyrazines isolation & purification
Rats
Rats, Sprague-Dawley
Apoptosis drug effects
Ligusticum chemistry
Photoreceptor Cells drug effects
Pyrazines pharmacology
Retina metabolism
Retina pathology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0513-4870
- Volume :
- 40
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Yao xue xue bao = Acta pharmaceutica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 16268501