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Arabidopsis nitric oxide synthase1 is targeted to mitochondria and protects against oxidative damage and dark-induced senescence.

Authors :
Guo FQ
Crawford NM
Source :
The Plant cell [Plant Cell] 2005 Dec; Vol. 17 (12), pp. 3436-50. Date of Electronic Publication: 2005 Nov 04.
Publication Year :
2005

Abstract

The Arabidopsis thaliana protein nitric oxide synthase1 (NOS1) is needed for nitric oxide (NO) synthesis and signaling during defense responses, hormonal signaling, and flowering. The cellular localization of NOS1 was examined because it is predicted to be a mitochondrial protein. NOS1-green fluorescent protein fusions were localized by confocal microscopy to mitochondria in roots. Isolated mitochondria from leaves of wild-type plants supported Arg-stimulated NO synthesis that could be inhibited by NOS inhibitors and quenched by a NO scavenger; this NOS activity is absent in mitochondria isolated from nos1 mutant plants. Because mitochondria are a source of reactive oxygen species (ROS), which participate in senescence and programmed cell death, these parameters were examined in the nos1 mutant. Dark-induced senescence of detached leaves and intact plants progressed more rapidly in the mutant compared with the wild type. Hydrogen peroxide, superoxide anion, oxidized lipid, and oxidized protein levels were all higher in the mutant. These results demonstrate that NOS1 is a mitochondrial NOS that reduces ROS levels, mitigates oxidative damage, and acts as an antisenescence agent.

Details

Language :
English
ISSN :
1040-4651
Volume :
17
Issue :
12
Database :
MEDLINE
Journal :
The Plant cell
Publication Type :
Academic Journal
Accession number :
16272429
Full Text :
https://doi.org/10.1105/tpc.105.037770