Back to Search
Start Over
Analysis of Poly-beta-Hydroxybutyrate in Rhizobium japonicum Bacteroids by Ion-Exclusion High-Pressure Liquid Chromatography and UV Detection.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 1983 Dec; Vol. 46 (6), pp. 1339-44. - Publication Year :
- 1983
-
Abstract
- Ion-exclusion high-pressure liquid chromatography (HPLC) was used to measure poly-beta-hydroxybutyrate (PHB) in Rhizobium japonicum bacteroids. The products in the acid digest of PHB-containing material were fractionated by HPLC on Aminex HPX-87H ion-exclusion resin for organic acid analysis. Crotonic acid formed from PHB during acid digestion was detected by its intense absorbance at 210 nm. The Aminex-HPLC method provides a rapid and simple chromatographic technique for routine analysis of organic acids. Results of PHB analysis by Aminex-HPLC were confirmed by gas chromatography and spectrophotometric analysis.
Details
- Language :
- English
- ISSN :
- 0099-2240
- Volume :
- 46
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 16346443
- Full Text :
- https://doi.org/10.1128/aem.46.6.1339-1344.1983