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Biodegradable polyurethane cytocompatibility to fibroblasts and staphylococci.

Biodegradable polyurethane cytocompatibility to fibroblasts and staphylococci.

Authors :
Harris LG
Gorna K
Gogolewski S
Richards RG
Source :
Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2006 May; Vol. 77 (2), pp. 304-12.
Publication Year :
2006

Abstract

Biodegradable polyurethanes have potential for use as implantable devices (orthopedic, maxillofacial, cardiovascular, wound dressing and plastic surgery) because of their controllable elasticity, and the possibility of changing their chemistry and structure. Studying bacterial and cell adhesion to polyurethanes helps to determine surface cytocompatibility and suitability for in vivo trials. Staphylococcus aureus, Staphylococcus epidermidis and hTERT human fibroblast cells were used to determine the cytocompatibility of experimental biodegradable polyurethanes (PUs) with different hydrophobic-to-hydrophilic (pho:phi) content ratios (100% pho, 70% pho, and 30% pho). Poly(L/DL-lactide) 70/30% (PLDL) and Thermanox were used as control surfaces. Surface characterization using noncontact profilometry, contact angles, and scanning electron microscopy (SEM) showed that the three PU surfaces, PLDL, and Thermanox have different properties. On the 100% PU and 30% PU surfaces, hTERT cells spread less in comparison to the 70% PU, PLDL, and Thermanox surfaces. The adsorption of fibronectin to the surfaces had no effect on the adhesion and spreading of hTERT cells when compared to the uncoated surfaces. The trend for S. aureus was the most adhered on the 70% PU and 30% PU, then Thermanox, followed by 100% PU and PLDL, respectively. The amount of S. epidermidis adhesion followed the trend of the most on 70% PU, then 100% PU, then 30% PU and PLDL, and the least on Thermanox. These results suggest that the 70% PU surface is cytocompatible to hTERT fibroblasts, while the 100% PU and 30% PU were not. All surfaces encouraged S. aureus and S. epidermidis colonization, particularly the 70% PU.<br /> (Copyright (c) 2006 Wiley Periodicals, Inc.)

Details

Language :
English
ISSN :
1549-3296
Volume :
77
Issue :
2
Database :
MEDLINE
Journal :
Journal of biomedical materials research. Part A
Publication Type :
Academic Journal
Accession number :
16400656
Full Text :
https://doi.org/10.1002/jbm.a.30621