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Two-dimensional electrophoretic profiling of atopic dermatitis in primary cultured keratinocytes from patients.

Authors :
Park YD
Jang HS
Kim SY
Ko SK
Lyou YJ
Lee DY
Paik YK
Yang JM
Source :
Proteomics [Proteomics] 2006 Feb; Vol. 6 (4), pp. 1362-70.
Publication Year :
2006

Abstract

Recently, we reported altered protein expression in primary cultured fibroblasts from atopic dermatitis (AD) patients. As a sequential study, we conducted proteomic analysis of primary keratinocytes derived from AD patients to further identify AD-related proteins. Three pH ranges, 4-7, 6-9, and 7-11, were used to profile the altered protein expression in AD. We obtained 46 candidate spots from the 2-D gel image analysis: 18 proteins were up-regulated and 27 proteins were down-regulated. Among the several important candidate proteins, NCC27 showed the same profile of a defect in PTM in both AD-derived keratinocytes and fibroblasts. On the basis of current and previous reports, real-time PCR was performed on select candidate genes to compare RNA and protein expression levels in AD-derived keratinocytes and fibroblasts. Our results provide new clues to aid in understanding the mechanism of atopic alterations in keratinocytes and suggest new AD-associated proteins that are important in AD pathogenesis.

Details

Language :
English
ISSN :
1615-9853
Volume :
6
Issue :
4
Database :
MEDLINE
Journal :
Proteomics
Publication Type :
Academic Journal
Accession number :
16402356
Full Text :
https://doi.org/10.1002/pmic.200500277