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Lysine deficiency and feed restriction independently alter cationic amino acid transporter expression in chickens (Gallus gallus domesticus).
- Source :
-
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [Comp Biochem Physiol A Mol Integr Physiol] 2006 Feb; Vol. 143 (2), pp. 218-27. Date of Electronic Publication: 2006 Jan 10. - Publication Year :
- 2006
-
Abstract
- The effect of a lysine-deficient diet on cationic amino acid transporter (CAT1-3) mRNA expression was determined in broiler chickens. Chicks consumed a lysine-adequate (LA; 1.3% lysine) or lysine-deficient (LD; 0.7% lysine) diet. Pair-fed chicks consumed the LA diet in an amount equal to that consumed by LD chicks during the previous day (PLA). CAT 1-3 mRNA expression in the liver, pectoralis and bursa of LD chicks were lower than that of LA and PLA chicks (P<0.05), and levels were not detectable in LD chick thymus. High affinity CAT mRNA expression in isolated bursacytes was 16-fold higher in LD chicks than that of LA chicks (P<0.001). Thymocyte high affinity CAT mRNA expression was 5-fold lower than that of LA chicks (P<0.05). The summed amount of high affinity CAT-1 and CAT-3 mRNA expression in chicks fed a lysine adequate diet was highly correlated (r2=0.51; P<0.001) to a tissue's growth during a lysine deficiency or feed restriction. In the thymus and bursa of LD chicks, CAT mRNA levels differed between resident lymphocytes and their surrounding tissues. By expressing high affinity CAT isoforms, developing lymphocytes may have a greater ability to obtain lysine than their surrounding tissue during a lysine deficiency.
- Subjects :
- Actins metabolism
Amino Acids metabolism
Amino Acids, Essential
Animal Feed
Animals
Chickens
Chloramphenicol O-Acetyltransferase metabolism
Corticosterone blood
DNA Primers chemistry
Gene Expression
Lymphocytes metabolism
Lysine chemistry
Male
Nutritional Requirements
Polymerase Chain Reaction
Protein Isoforms
RNA, Messenger metabolism
Reverse Transcriptase Polymerase Chain Reaction
Thymus Gland cytology
Thymus Gland metabolism
Amino Acid Transport Systems, Basic biosynthesis
Lysine deficiency
Lysine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-6433
- Volume :
- 143
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
- Publication Type :
- Academic Journal
- Accession number :
- 16406639
- Full Text :
- https://doi.org/10.1016/j.cbpa.2005.11.019