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Determination of tetracycline, chlortetracycline and oxytetracycline by flow injection with inhibitory chemiluminescence detection using copper(II) as a probe ion.
- Source :
-
Luminescence : the journal of biological and chemical luminescence [Luminescence] 2006 Mar-Apr; Vol. 21 (2), pp. 106-11. - Publication Year :
- 2006
-
Abstract
- This paper reports an indirect flow-injection (FI) method for the determination of the tetracycline drugs (TCs), tetracycline (TC), chlortetracycline (CTC) and oxytetracycline (OTC), using copper(II) as a probe ion. The method was based on the inhibition caused by these TCs to the copper(II)-catalysed chemiluminescence (CL) reaction between luminol and H(2)O(2). The CL reaction was induced on-line and injection of the sample produced negative peaks as a result of the copper(II) complexation or displacement by the analytes. The height of the peaks was proportional to the drug concentration in the sample. The choice of the catalyst ion, the concentration of luminol, H(2)O(2) and copper(II) are discussed. The linear range was 3.6 x 10(-8)-1.0 x 10(-5), 1.1 x 10(-7)-1.0 x 10(-5) and 1.9 x 10(-7)-1.0 x 10(-5) mol/L for TC, CTC and OTC, respectively. The detection limit was 5.0 x 10(-9) mol/L for TC, 1.0 x 10(-8) mol/L for CTC and 2.0 x 10(-8) mol/L for OTC (3sigma), respectively. The method was applied to the determination of TCs in pharmaceutical preparations and human urine with recoveries in the range 95-105%.
- Subjects :
- Catalysis
Flow Injection Analysis instrumentation
Flow Injection Analysis methods
Humans
Hydrogen Peroxide chemistry
Hydrogen-Ion Concentration
Luminol chemistry
Sensitivity and Specificity
Chlortetracycline urine
Copper chemistry
Luminescence
Luminescent Measurements
Oxytetracycline urine
Tetracycline urine
Subjects
Details
- Language :
- English
- ISSN :
- 1522-7235
- Volume :
- 21
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Luminescence : the journal of biological and chemical luminescence
- Publication Type :
- Academic Journal
- Accession number :
- 16416504
- Full Text :
- https://doi.org/10.1002/bio.893