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Arabidopsis Bax inhibitor-1 functions as an attenuator of biotic and abiotic types of cell death.
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 2006 Mar; Vol. 45 (6), pp. 884-94. - Publication Year :
- 2006
-
Abstract
- Programmed cell death (PCD) is a common process in eukaryotes during development and in response to pathogens and stress signals. Bax inihibitor-1 (BI-1) is proposed to be a cell death suppressor that is conserved in both animals and plants, but the physiological importance of BI-1 and the impact of its loss of function in plants are still unclear. In this study, we identified and characterized two independent Arabidopsis mutants with a T-DNA insertion in the AtBI1 gene. The phenotype of atbi1-1 and atbi1-2, with a C-terminal missense mutation and a gene knockout, respectively, was indistinguishable from wild-type plants under normal growth conditions. However, these two mutants exhibit accelerated progression of cell death upon infiltration of leaf tissues with a PCD-inducing fungal toxin fumonisin B1 (FB1) and increased sensitivity to heat shock-induced cell death. Under these conditions, expression of AtBI1 mRNA was up-regulated in wild-type leaves prior to the activation of cell death, suggesting that increase of AtBI1 expression is important for basal suppression of cell death progression. Over-expression of AtBI1 transgene in the two homozygous mutant backgrounds rescued the accelerated cell death phenotypes. Together, our results provide direct genetic evidence for a role of BI-1 as an attenuator for cell death progression triggered by both biotic and abiotic types of cell death signals in Arabidopsis.
- Subjects :
- Amino Acid Sequence
Arabidopsis anatomy & histology
Arabidopsis genetics
Arabidopsis Proteins genetics
Base Sequence
DNA, Bacterial genetics
Fumonisins pharmacology
Hot Temperature
Membrane Proteins genetics
Molecular Sequence Data
Mutagenesis, Insertional
Mutation
Phenotype
Plant Leaves anatomy & histology
Plant Leaves drug effects
Plant Leaves metabolism
Signal Transduction genetics
Transgenes
Up-Regulation
Apoptosis
Arabidopsis metabolism
Arabidopsis Proteins physiology
Membrane Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0960-7412
- Volume :
- 45
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 16507080
- Full Text :
- https://doi.org/10.1111/j.1365-313X.2006.02654.x