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Apoptosis of lymphocytes in allograft in a rat liver transplantation model induced by resveratrol.
- Source :
-
Pharmacological research [Pharmacol Res] 2006 Jul; Vol. 54 (1), pp. 19-23. Date of Electronic Publication: 2006 Mar 03. - Publication Year :
- 2006
-
Abstract
- Aim: To study the effect of resveratrol (RES) on the apoptosis of lymphocytes in allograft in a rat liver transplantation model.<br />Methods: Male Sprague-Dawley (SD) rats were selected as donors and male Wistar rats as recipients for a rejection model. The recipients were divided into four groups after orthotopic liver transplantation (OLTx). In the RES A, B, and C groups, RES was given intraperitoneally once a day (25, 50, and 100 mgkg(-1), respectively) after OLTx, whereas in the control group vehicle buffer was given intraperitoneally once a day. The survival period, lymphocytes apoptosis, expressions of Bcl-2/Bax proteins in lymphocytes, and histopathological findings were then compared among these groups.<br />Results: The mean survival period after OLTx in RES C group was significantly longer than that in control group (P < 0.05). On the seventh post-transplant day, the apoptosis index (AI) of lymphocytes in portal area and the positive rate of Bax protein in lymphocytes in RES C group were significantly increased in comparison with those in control group (both P < 0.05), whereas there is no obvious difference in the expression of Bcl-2 protein in lymphocytes between the control group and various drug groups (all P < 0.05), and a histological examination revealed apparent difference in the severity of rejection between the RES C group and control group (P < 0.05).<br />Conclusion: RES has an immunosuppressive effect on lymphocytes under allograft rejection in rat. Inducing apoptosis of lymphocytes and upregulating the ratio of Bax/bcl-2 proteins in lymphocytes in allograft liver may be part of the mechanisms.
- Subjects :
- Animals
Graft Rejection
Immunosuppressive Agents pharmacology
Liver Transplantation pathology
Male
Proto-Oncogene Proteins c-bcl-2 biosynthesis
Rats
Rats, Sprague-Dawley
Rats, Wistar
Resveratrol
T-Lymphocytes immunology
T-Lymphocytes metabolism
Transplantation, Homologous
bcl-2-Associated X Protein biosynthesis
Apoptosis drug effects
Liver Transplantation immunology
Stilbenes pharmacology
T-Lymphocytes drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1043-6618
- Volume :
- 54
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Pharmacological research
- Publication Type :
- Academic Journal
- Accession number :
- 16517175
- Full Text :
- https://doi.org/10.1016/j.phrs.2006.01.011