[Recombinant non-structural 3A, 3B and 3AB proteins of foot-and-mouth disease virus: use for differentiation of vaccinated and infected cattle].

Authors :: Iakovleva AS
Shcherbakov AV
Kan'shina AV
Mudrak NS
Fomina TA
Source :: Molekuliarnaia biologiia [Mol Biol (Mosk)] 2006 Jan-Feb; Vol. 40 (1), pp. 165-71.
Publication Year :: 2006
Abstract: Recombinant proteins 3A, 3B and 3AB were obtained by expression in Escherichia coli and purified by metal-chelate chromatography. The proteins were used as antigens in indirect ELISA to differentiate vaccinated and infected cattle. While testing 200 sera from cattle 3A-ELISA was more sensitive and specific than 3B- and 3AB-ELISA. Compared with "Chekit FMD-3ABC", 3A-ELISA showed the same level of specificity and higher level of sensitivity.

Subjects :: Animals
Antigens, Viral immunology
Carrier State diagnosis
Carrier State virology
Cattle
Enzyme-Linked Immunosorbent Assay
Foot-and-Mouth Disease diagnosis
Foot-and-Mouth Disease prevention & control
Foot-and-Mouth Disease Virus isolation & purification
Recombinant Proteins immunology
Carrier State veterinary
Foot-and-Mouth Disease virology
Foot-and-Mouth Disease Virus immunology
Viral Nonstructural Proteins immunology
Viral Vaccines immunology

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