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Optoinjection for efficient targeted delivery of a broad range of compounds and macromolecules into diverse cell types.
- Source :
-
Journal of biomedical optics [J Biomed Opt] 2006 Jan-Feb; Vol. 11 (1), pp. 014034. - Publication Year :
- 2006
-
Abstract
- Efficient delivery of compounds and macromolecules into living cells is essential in many fields including basic research, applied drug discovery, and clinical gene therapy. Unfortunately, current delivery methods, such as cationic lipids and electroporation, are limited by the types of macromolecules and cells that can be employed, poor efficiency, and/or cell toxicity. To address these issues, novel methods were developed based on laser-mediated delivery of macromolecules into cells through optoinjection. An automated high-throughput instrument, the laser-enabled analysis and processing (LEAP) system, was utilized to elucidate and optimize several parameters that influence optoinjection efficiency and toxicity. Techniques employing direct cell irradiation (i.e., targeted to specific cell coordinates) and grid-based irradiation (i.e., without locating individual cells) were both successfully developed. With both techniques, it was determined that multiple, sequential low radiant exposures produced more favorable results than a single high radiant exposure. Various substances were efficiently optoinjected--including ions, small molecules, dextrans, siRNAs (small interfering RNAs), plasmids, proteins, and semiconductor nanocrystals--into numerous cell types. Notably, cells refractory to traditional delivery methods were efficiently optoinjected with lower toxicity. We establish the broad utility of optoinjection, and furthermore, are the first to demonstrate its implementation in an automated, high-throughput manner.
- Subjects :
- Animals
Cell Line
Cricetinae
Dose-Response Relationship, Radiation
Humans
Lasers
Mice
Radiation Dosage
Species Specificity
Stress, Mechanical
Cell Membrane metabolism
Cell Membrane radiation effects
Cell Membrane Permeability radiation effects
Drug Delivery Systems methods
Microinjections methods
Pharmaceutical Preparations administration & dosage
Pharmacokinetics
Subjects
Details
- Language :
- English
- ISSN :
- 1083-3668
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of biomedical optics
- Publication Type :
- Academic Journal
- Accession number :
- 16526911
- Full Text :
- https://doi.org/10.1117/1.2168148