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[Apoptosis of mesenchymal cell line MBA-1 induced by core binding factor alpha 1].
- Source :
-
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences [Zhong Nan Da Xue Xue Bao Yi Xue Ban] 2006 Feb; Vol. 31 (1), pp. 14-8. - Publication Year :
- 2006
-
Abstract
- Objective: To investigate the effect of two core binding factors alpha 1 (Cbfa1) isfroms (Cbfa1/P56 and Cbfa1/P57) on the apoptosis of mesenchymal cell line MBA-1.<br />Methods: The two Cbfal isfroms were transiently transfected into MBA-1 cells, then the changes of apoptosis rate were observed by flow cytometer. The protein expressions of Cbfa1, Bax, Bcl-2, caspase-3, and caspase-9, cytochrome-C and TNF-alpha were determined by Western immunoblot.<br />Results: After the transient transfection with the two isforms of Cbfa1, MBA-1, the cells apoptotic rates increased, and the ratio of Bax/Bcl-2, the expressions of cytochrome-C, caspase-3, caspase-9, and TNF-alpha were significantly increased.<br />Conclusion: Cbfa1 can promote the apoptosis in mesenchymal cell line MBA-1. Bax/Bcl-2, cytochrome-C, caspase-9, caspase-3, and TNF-alpha are also involved in the apoptosis pathway.
- Subjects :
- Animals
Caspase 9
Caspases metabolism
Cell Line
Cytochromes c metabolism
Mice
Protein Isoforms
Proto-Oncogene Proteins c-bcl-2 metabolism
bcl-2-Associated X Protein metabolism
Apoptosis physiology
Bone Marrow Cells cytology
Core Binding Factor alpha Subunits pharmacology
Mesenchymal Stem Cells cytology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1672-7347
- Volume :
- 31
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 16562667