An efficient technique for obtaining sequences flanking inserted retroviruses.

Authors :: Huckaby CS
Kouri RE
Lane MJ
Peshick SM
Carroll WT
Henderson SM
Faldasz BD
Waterbury PG
Vournakis JN
Source :: Genetic analysis, techniques and applications [Genet Anal Tech Appl] 1991 Aug; Vol. 8 (5), pp. 151-8.
Publication Year :: 1991
Abstract: Genomic mapping studies frequently employ retrovirus-mediated transfer of dominant selectable markers to specific target chromosomes. DNA probes containing sequences adjacent to inserted proviruses are valuable mapping tools in such studies. We have implemented a strategy for amplification of chromosomal sequences flanking the 5' LTR of MoMuLV-based vectors. Probes derived from these amplification products successfully differentiated murine versus human proviral localization in retrovirus-infected mouse-human chromosome 17q hybrid cells.