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PslD is a secreted protein required for biofilm formation by Pseudomonas aeruginosa.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 2006 Apr; Vol. 72 (4), pp. 3066-8. - Publication Year :
- 2006
-
Abstract
- The function of pslD, which is part of the psl operon from Pseudomonas aeruginosa, was investigated in this study. The psl operon is involved in exopolysaccharide biosynthesis and biofilm formation. An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1 which was deficient in the formation of differentiated biofilms was generated. Expression of only the pslD gene coding region restored the wild-type phenotype. A C-terminal, hexahistidine tag fusion enabled the identification of PslD. LacZ and PhoA translational fusions with PslD indicated that PslD is a secreted protein required for biofilm formation, presumably via its role in exopolysaccharide export.
- Subjects :
- Bacterial Outer Membrane Proteins chemistry
Bacterial Outer Membrane Proteins genetics
Microscopy, Fluorescence
Mutation
Periplasm metabolism
Pseudomonas aeruginosa genetics
Pseudomonas aeruginosa metabolism
Bacterial Outer Membrane Proteins metabolism
Biofilms growth & development
Gene Expression Regulation, Bacterial
Pseudomonas aeruginosa growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 0099-2240
- Volume :
- 72
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 16598021
- Full Text :
- https://doi.org/10.1128/AEM.72.4.3066-3068.2006