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CD16 and CR3 receptors distinguish between the two mechanisms of tumour cytotoxicity in neutrophils.
- Source :
-
British journal of haematology [Br J Haematol] 1991 Oct; Vol. 79 (2), pp. 170-6. - Publication Year :
- 1991
-
Abstract
- Previous studies have suggested that phorbol ester-activated neutrophils kill both antibody non-coated and antibody-coated K562 target cells. In this report the contribution of the receptors Fc gamma III (CD16) and CR3 (CD11b/CD18) in the lytic process was investigated. In neutrophils CD16 and CR3 are up-regulated by the phorbol ester up to 4 and 10 times, respectively. As expected, lysis of non-immunized K562 targets is not affected by the treatment of neutrophils with anti CD16, AB8.28, whereas lysis of immunized targets is decreased by 50%. In addition, the interaction of CD16 and AB8.28 induces calcium mobilization and increases granule secretion. Surprisingly, the simultaneous binding of AB8.28 and anti-CR3 OKM1 to neutrophils completely abolishes the lysis of antibody-coated targets. Unlike CD16, CR3 does not possess a functional role and binding of OKM1 to CR3 does not affect cytotoxicity of immunized K562 targets, but it blocks lysis of non-coated target almost completely, indicating a function as adhesion protein for CR3. These studies demonstrate a distinct role of CD16 and CR3 in mediating antibody-dependent and antibody-independent cellular cytotoxicity, respectively.
- Subjects :
- Antibodies, Monoclonal immunology
Antigen-Antibody Reactions
Calcium metabolism
Cell Line
Cytotoxicity Tests, Immunologic
Humans
Leukemia immunology
Neutrophils metabolism
Receptors, IgG
Superoxides metabolism
Antibody-Dependent Cell Cytotoxicity immunology
Antigens, CD immunology
Antigens, Differentiation immunology
Macrophage-1 Antigen immunology
Neutrophils immunology
Receptors, Fc immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0007-1048
- Volume :
- 79
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- British journal of haematology
- Publication Type :
- Academic Journal
- Accession number :
- 1659860
- Full Text :
- https://doi.org/10.1111/j.1365-2141.1991.tb04518.x