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Amplification of JC virus DNA from brain and cerebrospinal fluid of patients with progressive multifocal leukoencephalopathy.
- Source :
-
Neurology [Neurology] 1991 Dec; Vol. 41 (12), pp. 1967-71. - Publication Year :
- 1991
-
Abstract
- We have established an assay to detect JC viral DNA in brain tissue and CSF of patients with progressive multifocal leukoencephalopathy (PML) and amplified a 106-base pair segment of the gene encoding JC viral large T antigen by polymerase chain reaction (PCR) from the brains of eight patients with PML and from one of six CSF samples. Brain and CSF of patients without PML did not yield JC virus-specific DNA amplification products. Southern analysis documented that the sequences of the 106-base pair PCR product and JC viral DNA are identical. This assay can be used in the diagnosis of PML and to investigate the biology of JC virus.
- Subjects :
- Base Sequence
Blotting, Southern
Brain microbiology
DNA, Viral cerebrospinal fluid
Humans
JC Virus genetics
Molecular Sequence Data
Oligonucleotides
Polymerase Chain Reaction
DNA, Viral analysis
JC Virus isolation & purification
Leukoencephalopathy, Progressive Multifocal microbiology
Tumor Virus Infections microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0028-3878
- Volume :
- 41
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Neurology
- Publication Type :
- Academic Journal
- Accession number :
- 1660572
- Full Text :
- https://doi.org/10.1212/wnl.41.12.1967