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Stimulation of phospholipase C activity by insulin is mediated by both isotypes of the human insulin receptor.

Authors :
Kellerer M
Machicao F
Seffer E
Mushack J
Ullrich A
Häring HU
Source :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1991 Dec 16; Vol. 181 (2), pp. 566-72.
Publication Year :
1991

Abstract

The human insulin receptor exists in two isoforms, HIR-A and HIR-B. We studied whether both insulin receptor isotypes are able to mediate an insulin signal to phospholipase C. Plasma membranes were prepared from rat-1 fibroblasts transfected either with HIR-A or HIR-B and insulin stimulated PIP-hydrolysis was determined. We found that insulin stimulates PIP-hydrolysis in a similar dose dependent manner and to a similar extent in plasma membranes expressing HIR-A and HIR-B. These data suggest that both receptor isoforms are equally able to activate phospholipase-C.

Details

Language :
English
ISSN :
0006-291X
Volume :
181
Issue :
2
Database :
MEDLINE
Journal :
Biochemical and biophysical research communications
Publication Type :
Academic Journal
Accession number :
1661583
Full Text :
https://doi.org/10.1016/0006-291x(91)91227-4