Back to Search
Start Over
Differential effects of antigens from L. braziliensis isolates from disseminated and cutaneous leishmaniasis on in vitro cytokine production.
- Source :
-
BMC infectious diseases [BMC Infect Dis] 2006 Apr 25; Vol. 6, pp. 75. Date of Electronic Publication: 2006 Apr 25. - Publication Year :
- 2006
-
Abstract
- Background: Disseminated leishmaniasis is an emerging infectious disease, mostly due to L. braziliensis, which has clinical and histopathological features distinct from cutaneous leishmaniasis.<br />Methods: In the current study we evaluated the in vitro production of the cytokines IFN-gamma, TNF-alpha, IL-5 and IL-10 by peripheral blood mononuclear cells (PBMC) from 15 disseminated leishmaniasis and 24 cutaneous leishmaniasis patients upon stimulation with L. braziliensis antigens genotyped as disseminated leishmaniasis or cutaneous leishmaniasis isolates.<br />Results: Regardless of the source of L. braziliensis antigens, PBMC from cutaneous leishmaniasis patients produced significantly higher IFN-gamma than PBMC from disseminated leishmaniasis patients. Levels of TNF-alpha by PBMC from cutaneous leishmaniasis patients were significantly higher than disseminated leishmaniasis patients only when stimulated by genotyped cutaneous leishmaniasis antigens. The levels of IL-5 and IL-10 production by PBMC were very low and similar in PBMCs from both disseminated leishmaniasis and cutaneous leishmaniasis patients. The immune response of each patient evaluated by the two L. braziliensis antigens was assessed in a paired analysis in which we showed that L. braziliensis genotyped as disseminated leishmaniasis isolate was more potent than L. braziliensis genotyped as cutaneous leishmaniasis isolate in triggering IFN-gamma and TNF-alpha production in both diseases and IL-5 only in cutaneous leishmaniasis patients.<br />Conclusion: This study provides evidence that antigens prepared from genotypically distinct strains of L. braziliensis induce different degrees of immune response. It also indicates that both parasite and host play a role in the outcome of L. braziliensis infection.
- Subjects :
- Adolescent
Adult
Animals
Antigens, Protozoan pharmacology
Cells, Cultured
Child
Cytokines drug effects
Cytokines immunology
Enzyme-Linked Immunosorbent Assay
Gene Expression Regulation
Humans
Interferon-gamma analysis
Interferon-gamma biosynthesis
Interleukin-10 analysis
Interleukin-10 biosynthesis
Interleukin-5 analysis
Interleukin-5 biosynthesis
Leishmaniasis, Cutaneous immunology
Leishmaniasis, Cutaneous parasitology
Leukocytes, Mononuclear drug effects
Middle Aged
Tumor Necrosis Factor-alpha analysis
Tumor Necrosis Factor-alpha biosynthesis
Antigens, Protozoan immunology
Cytokines biosynthesis
Leishmania braziliensis immunology
Leishmaniasis, Diffuse Cutaneous immunology
Leishmaniasis, Diffuse Cutaneous parasitology
Leukocytes, Mononuclear immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2334
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- BMC infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 16638143
- Full Text :
- https://doi.org/10.1186/1471-2334-6-75