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Identification and partial purification of serologically defined Boophilus microplus larval antigens by natural ectoparasite exposure.

Authors :
Pruett JH
Untalan PM
Davey RB
Source :
Veterinary parasitology [Vet Parasitol] 2006 Aug 31; Vol. 140 (1-2), pp. 148-57. Date of Electronic Publication: 2006 May 02.
Publication Year :
2006

Abstract

In an effort to identify life-stage specific Boophilus microplus proteins that elicit a humoral response in cattle, soluble proteins were extracted from 10- to 14-day-old larvae and subsequently fractionated by size-exclusion chromatography and reverse-phase high pressure liquid chromatography. Several antigens were identified by Western blotting as potentially shared with other ixodid tick species since antibodies to these proteins were present in sera of calves not previously exposed to B. microplus. Six putative B. microplus-specific antigens were identified by antibodies in the sera of calves repeatedly exposed to B. microplus larvae. One of the antigens, a 19.1 kDa protein, was used in the development of a diagnostic kELISA for previous exposure to B. microplus. The 19.1 kDa protein did not have tryptic protease activity or inhibit bovine trypsin activity, but appeared to be allergenic in that a partially pure fraction elicited immediate-type hypersensitivity responses in calves previously exposed to B. microplus.

Details

Language :
English
ISSN :
0304-4017
Volume :
140
Issue :
1-2
Database :
MEDLINE
Journal :
Veterinary parasitology
Publication Type :
Academic Journal
Accession number :
16650598
Full Text :
https://doi.org/10.1016/j.vetpar.2006.03.017