Improved multiplex PCR method for the rapid detection of beta-lactamase genes in Escherichia coli of animal origin.

Authors :: Pomba C
Mendonça N
Costa M
Louro D
Baptista B
Ferreira M
Correia JD
Caniça M
Source :: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2006 Sep; Vol. 56 (1), pp. 103-6. Date of Electronic Publication: 2006 May 05.
Publication Year :: 2006
Abstract: We developed 2 variants, A and B, of a multiplex polymerase chain reaction method for detecting the beta-lactam resistance genes bla(TEM), bla(SHV), and bla(OXA) in 122 uropathogenic Escherichia coli animal strains. Method B yielded 98% specificity and 100% sensitivity, and method A yielded 100% and 89%, respectively. Variant B was more accurate (99%) than A (94%).

Subjects :: Animals
Cat Diseases microbiology
Cats
Dog Diseases microbiology
Dogs
Escherichia coli drug effects
Escherichia coli isolation & purification
Escherichia coli Infections genetics
Genes, Bacterial
Genotype
Swine
Swine Diseases microbiology
Urinary Tract Infections microbiology
Escherichia coli genetics
Escherichia coli Infections veterinary
Polymerase Chain Reaction methods
Urinary Tract Infections veterinary
beta-Lactam Resistance genetics

Full Text Access

Tools