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Analysis of maize brittle-1 alleles and a defective Suppressor-mutator-induced mutable allele.
- Source :
-
The Plant cell [Plant Cell] 1991 Dec; Vol. 3 (12), pp. 1337-48. - Publication Year :
- 1991
-
Abstract
- A mutant allele of the maize brittle-1 (bt1) locus, brittle-1-mutable (bt1-m), was shown genetically and molecularly to result from the insertion of a defective Suppressor-mutator (dSpm) transposable element. An Spm-hybridizing restriction enzyme fragment, which cosegregates with the bt1-m allele and is absent from wild-type revertants of bt1-m, was identified and cloned. Non-Spm portions of it were used as probes to identify wild-type (Bt1) cDNAs in an endosperm library. The 4.3-kb bt1-m genomic clone contains a 3.3-kb dSpm, which is inserted in an exon and is composed of Spm termini flanking non-Spm sequences. RNA gel blot analyses, using a cloned Bt1 cDNA probe, indicated that Bt1 mRNA is present in the endosperm of developing kernels and is absent from embryo or leaf tissues. Several transcripts are produced by bt1-m. The deduced translation product from a 1.7-kb Bt1 cDNA clone has an apparent plastid transit peptide at its amino terminus and sequence similarity to several mitochondrial inner-envelope translocator proteins, suggesting a possible role in amyloplast membrane transport.
- Subjects :
- Alleles
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA
DNA Transposable Elements
Membrane Proteins metabolism
Molecular Sequence Data
Phenotype
Plant Proteins metabolism
Sequence Homology, Amino Acid
Membrane Proteins genetics
Plant Proteins genetics
Suppression, Genetic
Zea mays genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1040-4651
- Volume :
- 3
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- The Plant cell
- Publication Type :
- Academic Journal
- Accession number :
- 1668652
- Full Text :
- https://doi.org/10.1105/tpc.3.12.1337