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[Value of combinational detection by IgH and IgL primers in improving detection rate of lymphoma gene in paraffin-embedded tissue].
- Source :
-
Ai zheng = Aizheng = Chinese journal of cancer [Ai Zheng] 2006 May; Vol. 25 (5), pp. 640-4. - Publication Year :
- 2006
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Abstract
- Background & Objective: Clonality detection through amplifying immunoglobulin heavy chain (IgH) by polymerase chain reaction (PCR) is a useful tool in diagnosis of lymphoma, but the false negative rate is high, especially in paraffin-embedded tissues. This study was to explore the value of tumor tissue microdissection and combinational detection of IgH and immunoglobulin light chain (Ig kappa or Ig lambda) in diagnosis of non-Hodgkin's lymphoma (NHL).<br />Methods: Two pairs of conventional primers for IgH and T-cell receptor gamma (TCRgamma), 2 novel designed pairs of primers for Ig kappa and Ig lambda were used to detect 58 paraffin-embedded blocks, which had been diagnosed by pathology and histochemistry. Of the 58 cases of lymph node tissues, 39 were B-cell lymphoma, 16 were T-cell lymphoma, and 3 were reactive proliferative lymph node tissue. Lymphoma cell lines DG75 and Jurkat were used as control.<br />Results: The positive rates of IgH primers (P1) and IgL primers (P kappa/P lambda) were 79.5% and 71.8% in the 39 cases of B-cell lymphoma (P>0.05), 6.3% and 12.5% in the 16 cases of T-cell lymphoma, respectively. The positive rate was greatly increased to 92.3% in combinationally detecting the primers for IgH and P kappa/P lambda. There was no positive detection among the reactive proliferative lymph node tissues.<br />Conclusion: B-cell lymphoma detection rate can be significantly improved by the combination of IgH and IgL gene rearrangement primers, which provides efficient assistant method for the diagnosis and differential diagnosis of lymphoma.
- Subjects :
- DNA Primers
Female
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Gene Rearrangement, B-Lymphocyte, Light Chain
Humans
Lymphoma, B-Cell diagnosis
Lymphoma, B-Cell immunology
Lymphoma, T-Cell diagnosis
Lymphoma, T-Cell immunology
Male
Paraffin Embedding
Polymerase Chain Reaction
Immunoglobulin Heavy Chains genetics
Immunoglobulin Light Chains genetics
Lymphoma, B-Cell genetics
Lymphoma, T-Cell genetics
Subjects
Details
- Language :
- Chinese
- Volume :
- 25
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Ai zheng = Aizheng = Chinese journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 16687090