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Molecular diagnosis of Burkitt's lymphoma.
- Source :
-
The New England journal of medicine [N Engl J Med] 2006 Jun 08; Vol. 354 (23), pp. 2431-42. - Publication Year :
- 2006
-
Abstract
- Background: The distinction between Burkitt's lymphoma and diffuse large-B-cell lymphoma is crucial because these two types of lymphoma require different treatments. We examined whether gene-expression profiling could reliably distinguish Burkitt's lymphoma from diffuse large-B-cell lymphoma.<br />Methods: Tumor-biopsy specimens from 303 patients with aggressive lymphomas were profiled for gene expression and were also classified according to morphology, immunohistochemistry, and detection of the t(8;14) c-myc translocation.<br />Results: A classifier based on gene expression correctly identified all 25 pathologically verified cases of classic Burkitt's lymphoma. Burkitt's lymphoma was readily distinguished from diffuse large-B-cell lymphoma by the high level of expression of c-myc target genes, the expression of a subgroup of germinal-center B-cell genes, and the low level of expression of major-histocompatibility-complex class I genes and nuclear factor-kappaB target genes. Eight specimens with a pathological diagnosis of diffuse large-B-cell lymphoma had the typical gene-expression profile of Burkitt's lymphoma, suggesting they represent cases of Burkitt's lymphoma that are difficult to diagnose by current methods. Among 28 of the patients with a molecular diagnosis of Burkitt's lymphoma, the overall survival was superior among those who had received intensive chemotherapy regimens instead of lower-dose regimens.<br />Conclusions: Gene-expression profiling is an accurate, quantitative method for distinguishing Burkitt's lymphoma from diffuse large-B-cell lymphoma.<br /> (Copyright 2006 Massachusetts Medical Society.)
- Subjects :
- Adolescent
Adult
Aged
Antineoplastic Combined Chemotherapy Protocols therapeutic use
Bayes Theorem
Burkitt Lymphoma mortality
Burkitt Lymphoma pathology
Child
Child, Preschool
Diagnosis, Differential
Female
Follow-Up Studies
Genes, MHC Class I
Genes, myc
Humans
In Situ Hybridization, Fluorescence
Lymphoma, B-Cell classification
Lymphoma, B-Cell diagnosis
Lymphoma, B-Cell mortality
Male
Middle Aged
NF-kappa B genetics
Oligonucleotide Array Sequence Analysis
Survival Analysis
Transcription, Genetic
Translocation, Genetic
Burkitt Lymphoma diagnosis
Burkitt Lymphoma genetics
Gene Expression
Gene Expression Profiling
Lymphoma, B-Cell genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1533-4406
- Volume :
- 354
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- The New England journal of medicine
- Publication Type :
- Academic Journal
- Accession number :
- 16760443
- Full Text :
- https://doi.org/10.1056/NEJMoa055759