Technical note: a rapid pulsed-field gel electrophoresis method for analysis of bifidobacteria.

Authors :: Briczinski EP
Roberts RF
Source :: Journal of dairy science [J Dairy Sci] 2006 Jul; Vol. 89 (7), pp. 2424-7.
Publication Year :: 2006
Abstract: Pulsed-field gel electrophoresis (PFGE) is a widely used and highly discriminatory molecular typing method that has been applied to bifidobacteria. However, published PFGE protocols used with bifidobacteria require between 5 and 7 d to complete. A rapid PFGE method was developed that can be completed within 24 h.

Subjects :: Polymerase Chain Reaction
Probiotics
RNA, Ribosomal, 16S genetics
Bifidobacterium classification
Bifidobacterium genetics
DNA, Bacterial analysis
Electrophoresis, Gel, Pulsed-Field methods

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