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Whole-cell recordings of calcium and potassium currents in acutely isolated smooth muscle cells.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2006 Jul 07; Vol. 12 (25), pp. 4086-8. - Publication Year :
- 2006
-
Abstract
- Aim: To record calcium and potassium currents in acutely isolated smooth muscle cells of mesenteric arterial branches in rats.<br />Methods: Smooth muscle cells were freshly isolated by collagenase digest and mechanical trituration with polished pipettes. Patch clamp technique in whole-cell mode was employed to record calcium and potassium currents.<br />Results: The procedure dissociated smooth muscle cells without impairing the electrophysiological characteristics of the cells. The voltage-gated Ca2+ and potassium currents were successfully recorded using whole-cell patch clamp configuration.<br />Conclusion: The method dissociates smooth muscle cells from rat mesenteric arterial branches. Voltage-gated channel currents can be recorded in this preparation.
Details
- Language :
- English
- ISSN :
- 1007-9327
- Volume :
- 12
- Issue :
- 25
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 16810766
- Full Text :
- https://doi.org/10.3748/wjg.v12.i25.4086