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Multispacer typing to study the genotypic distribution of Bartonella henselae populations.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2006 Jul; Vol. 44 (7), pp. 2499-506. - Publication Year :
- 2006
-
Abstract
- Bartonella henselae, a worldwide fastidious bacterium, has a feline reservoir and is pathogenic for humans. However, the relationship between human and cat isolates of B. henselae, as well as its population dynamics and geographic heterogeneity, is not fully understood, in part because of the absence of appropriate typing methods. Multilocus sequence typing (MLST), the most discriminatory genotyping method for B. henselae, identified seven genotypes and suggested that human isolates arose from a limited number of cat isolates. Herein, we estimated the discriminatory power of multispacer typing (MST) by studying 126 B. henselae cat isolates from various areas of Europe, Asia, and the United States. We identified the nine most variable intergenic spacers conserved by both B. henselae and Bartonella quintana genomes. By comparing the sequences obtained from these nine spacers for each studied isolate, we identified 39 MST genotypes. The distribution of isolates into MST genotypes matched their phylogenetic organization into four clusters. MST showed that European and Asian isolates were different, in contrast with American isolates, but failed to identify pandemic strains. Our study demonstrated that MST is a powerful method for genotyping B. henselae at the strain level and may serve in studying the population dynamics of this bacterium and understanding the relationships between cat and human isolates. Finally, we provide a free-access MST-Rick online software program (http://ifr48.timone.univ-mrs.fr/MST_BHenselae/mst) that investigators may use to compare their own MST sequences to our database.
- Subjects :
- Animals
Asia
Bartonella Infections microbiology
Bartonella Infections veterinary
Bartonella henselae isolation & purification
Bartonella quintana genetics
Cat Diseases microbiology
Cats
Cluster Analysis
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Intergenic chemistry
DNA, Intergenic genetics
Europe
Genotype
Molecular Sequence Data
Phylogeny
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
United States
Bacterial Typing Techniques
Bartonella henselae classification
Bartonella henselae genetics
Molecular Epidemiology methods
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 44
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 16825371
- Full Text :
- https://doi.org/10.1128/JCM.00498-06