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Oviductal protease and trypsin treatment enhance sperm-envelope interaction in Bufo arenarum coelomic eggs.
- Source :
-
Journal of experimental zoology. Part A, Comparative experimental biology [J Exp Zool A Comp Exp Biol] 2006 Oct 01; Vol. 305 (10), pp. 872-82. - Publication Year :
- 2006
-
Abstract
- We describe the morphological and biochemical changes in Bufo arenarum coelomic egg envelopes (CE) following passage through the oviduct. In this species, the transformation of the CE into the vitelline envelope (VE) leads to the acquisition of fertilizability and involves the cleavage of a glycoprotein component. Electrophoretic patterns indicate that a pars recta oviductal protease selectively hydrolyzes in vitro the 84 and the 55 kDa glycoproteins of the CE. During the CE to VE transformation, the relative concentrations of gp48, 42 and 39 kDa also change. In in vitro tests, sperm binding to envelope glycoprotein occurs when they are exposed to VE but not when treated with CE, and VE labeled glycoproteins bind to the head and mid piece of the sperm. The gp39 VE component has 100% identity with internal domains of the sequence deduced from ovarian cDNA for the homologous zona pellucida glycoprotein type C (ZPC) protein precursor in B. arenarum. The effects of trypsin as a substitute for oviductal protease were also examined. Trypsin selectively attacks the 84 and the 55 kDa glycoproteins without hydrolyzing other components and renders coelomic eggs fertilizable in a jelly water preparation. Therefore, trypsin can mimic in vitro the biological action of the oviductal protease. However, it does not wholly mimic the biological action of the oviduct which, in B. arenarum at least, exceeds a mere proteolytic effect. This fact was verified by the lower fertility rates and the abnormal embryo development found when trypsin-treated coelomic eggs were fertilized in vitro.<br /> (2006 Wiley-Liss, Inc.)
- Subjects :
- Amino Acid Sequence
Animals
Female
Fertility drug effects
Glycoproteins chemistry
Glycoproteins metabolism
In Vitro Techniques
Male
Molecular Sequence Data
Oviducts enzymology
Ovum drug effects
Ovum physiology
Ovum ultrastructure
Sperm-Ovum Interactions physiology
Spermatozoa drug effects
Spermatozoa physiology
Vitelline Membrane chemistry
Vitelline Membrane metabolism
Bufo arenarum physiology
Serine Endopeptidases pharmacology
Sperm-Ovum Interactions drug effects
Trypsin pharmacology
Vitelline Membrane drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1548-8969
- Volume :
- 305
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of experimental zoology. Part A, Comparative experimental biology
- Publication Type :
- Academic Journal
- Accession number :
- 16838345
- Full Text :
- https://doi.org/10.1002/jez.a.299