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In vivo CD4+ T-cell up-regulation and high dose side effects of refolded duck interleukin-2.
- Source :
-
Cytokine [Cytokine] 2006 Jun; Vol. 34 (5-6), pp. 297-302. Date of Electronic Publication: 2006 Aug 01. - Publication Year :
- 2006
-
Abstract
- The recombinant duck interleukin-2 (rduIL-2) monomer was firstly isolated under nature condition, and refolded by oxidization procedure. Refolded rduIL-2 monomer induced in vitro proliferation of Con A-stimulated duck splenocytes in a sensitive and dose-dependent manner, and up-regulated in vivo the amounts of CD4+ T cells with low dose of administration. However, high doses intermittent administration resulted in sever side effects in vivo, with typical lymphocytic interstitial pneumonitis and nephritis, and lymphocytic depletion in splenic corpuscle. Our findings might be beneficial to studies of both mechanism and applications in vivo of avian IL-2.
- Subjects :
- Animals
CD8-Positive T-Lymphocytes drug effects
CD8-Positive T-Lymphocytes metabolism
Cell Proliferation drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Ducks
Gene Expression
Immunophenotyping
Interleukin-2 isolation & purification
Interleukin-2 toxicity
Protein Folding
Recombinant Proteins genetics
Recombinant Proteins isolation & purification
Recombinant Proteins metabolism
Recombinant Proteins pharmacology
Up-Regulation drug effects
CD4-Positive T-Lymphocytes drug effects
CD4-Positive T-Lymphocytes metabolism
Interleukin-2 metabolism
Interleukin-2 pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1043-4666
- Volume :
- 34
- Issue :
- 5-6
- Database :
- MEDLINE
- Journal :
- Cytokine
- Publication Type :
- Academic Journal
- Accession number :
- 16884909
- Full Text :
- https://doi.org/10.1016/j.cyto.2006.06.006