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Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography.
- Source :
-
Journal of chromatography. A [J Chromatogr A] 2006 Sep 15; Vol. 1127 (1-2), pp. 298-301. Date of Electronic Publication: 2006 Aug 02. - Publication Year :
- 2006
-
Abstract
- A recycling counter-current chromatographic system was first set up with a high-speed counter-current chromatography instrument coupled with a column switching valve. This system was first successfully applied to the preparative separation of epimers, gambogic acid and epigambogic acid from Garcinia hanburyi using n-hexane-methanol-water (5:4:1, v/v/v) as the two-phase solvent system. As a result, 28.2 mg gambogic acid and 18.4 mg epigambogic acid were separated from 50 mg of mixture. Their purities were both above 97% as determined by HPLC. The chemical structures were then identified by their (1)H NMR and (13)C NMR spectra.
Details
- Language :
- English
- ISSN :
- 0021-9673
- Volume :
- 1127
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. A
- Publication Type :
- Academic Journal
- Accession number :
- 16887130
- Full Text :
- https://doi.org/10.1016/j.chroma.2006.07.044