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Dendritic cell-derived exosomes stimulate stronger CD8+ CTL responses and antitumor immunity than tumor cell-derived exosomes.
- Source :
-
Cellular & molecular immunology [Cell Mol Immunol] 2006 Jun; Vol. 3 (3), pp. 205-11. - Publication Year :
- 2006
-
Abstract
- Exosomes (EXO) derived from dendritic cells (DC) and tumor cells have been used to stimulate antitumor immune responses in animal models and in clinical trials. However, there has been no side-by-side comparison of the stimulatory efficiency of the antitumor immune responses induced by these two commonly used EXO vaccines. In this study, we selected to study the phenotype characteristics of EXO derived from a transfected EG7 tumor cells expressing ovalbumin (OVA) and OVA-pulsed DC by flow cytometry. We compared the stimulatory effect in induction of OVA-specific immune responses between these two types of EXO. We found that OVA protein-pulsed DCOVA-derived EXO (EXODC) can more efficiently stimulate naïve OVA-specific CD8+ T cell proliferation and differentiation into cytotoxic T lymphocytes in vivo, and induce more efficient antitumor immunity than EG7 tumor cell-derived EXO (EXOEG7). In addition, we elucidated the important role of the host DC in EXO vaccines that the stimulatory effect of EXO is delivered to T cell responses by the host DC. Therefore, DC-derived EXO may represent a more effective EXO-based vaccine in induction of antitumor immunity.
- Subjects :
- Animals
Cancer Vaccines immunology
Cell Line, Tumor
Cell Proliferation
Cells, Cultured
Cytotoxicity Tests, Immunologic
Dendritic Cells metabolism
Disease Models, Animal
Exocytosis
Immunity
Immunophenotyping
Lung Neoplasms immunology
Lung Neoplasms secondary
Lymphocyte Activation
Melanoma, Experimental immunology
Melanoma, Experimental secondary
Mice
Mice, Inbred C57BL
Mice, Knockout
Neoplasm Transplantation
Ovalbumin biosynthesis
Ovalbumin genetics
Ovalbumin immunology
T-Lymphocytes, Cytotoxic cytology
Thymoma metabolism
Transport Vesicles metabolism
Transport Vesicles transplantation
Cancer Vaccines therapeutic use
Dendritic Cells immunology
Lung Neoplasms therapy
Melanoma, Experimental therapy
T-Lymphocytes, Cytotoxic immunology
Thymoma immunology
Transport Vesicles immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1672-7681
- Volume :
- 3
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cellular & molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16893501