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Extracellular matrix remodeling--methods to quantify cell-matrix interactions.

Authors :
Abraham LC
Dice JF
Finn PF
Mesires NT
Lee K
Kaplan DL
Source :
Biomaterials [Biomaterials] 2007 Jan; Vol. 28 (2), pp. 151-61. Date of Electronic Publication: 2006 Aug 07.
Publication Year :
2007

Abstract

Tissue turnover during wound healing, regeneration or integration of biomedical materials depends on the rate and extent of materials trafficking into and out of cells involved in extracellular matrix (ECM) remodeling. To exploit these processes, we report the first model for matrix trafficking in which these issues are quantitatively assessed for cells grown on both native collagen (normal tissue) and denatured collagen (wound state) substrates. Human fibroblasts more rapidly remodeled denatured versus normal collagen type I to form new ECM. Fluxes to and from the cells from the collagen substrates and the formation of new ECM were quantified using radioactively labeled substrates. The model can be employed for the systematic and quantitative study of the impact of a broad range of physiological factors and disease states on tissue remodeling, integrating extracellular matrix structures and cell biology.

Details

Language :
English
ISSN :
0142-9612
Volume :
28
Issue :
2
Database :
MEDLINE
Journal :
Biomaterials
Publication Type :
Academic Journal
Accession number :
16893566
Full Text :
https://doi.org/10.1016/j.biomaterials.2006.07.001