Viability and molecular authentication of Coccidioides immitis strains from culture collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.

Authors :: Bezerra CC
de Lima RF
Lazera MS
Wanke B
Borba CM
Source :: Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2006 May-Jun; Vol. 39 (3), pp. 241-4.
Publication Year :: 2006
Abstract: Twenty Coccidioides immitis strains were evaluated. Only 5 of the 20 strains kept under mineral oil maintained their viability while all 5 subcultures preserved in water remained viable and none of the 13 subcultures kept in soil were viable. A 519 bp PCR product from the csa gene confirmed the identity of the strains.

Subjects :: Brazil
Coccidioides classification
Polymerase Chain Reaction
Time Factors
Coccidioides genetics
Microbial Viability
Preservation, Biological methods

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