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[Reactivity of a novel monoclonal antibody ZCH-7-2D3 on leukemia cells and its clinical significance].
- Source :
-
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences [Zhejiang Da Xue Xue Bao Yi Xue Ban] 2006 Jul; Vol. 35 (4), pp. 390-3. - Publication Year :
- 2006
-
Abstract
- Objective: To analyze the reactive pattern and its clinical significance of ZCH-7-2D3 monoclonal antibody on leukemia cells.<br />Methods: Bone marrow samples from 100 leukemia patients (male 59: female 41, 34 cases of children and 66 adults, aged 11 months - 77 year) were collected. A CD45 gating strategy and multi-parameter flow cytometry were used to analyze the leukemia cells.<br />Result: The positive rate (10/31) of 2D3 antibody on acute lymphocytic leukemia (ALL) patients was significantly lower than that (39/55) of acute myelogeneous leukemia (AML) (P <0.01). 2D3 was positive for all three cases of B/myeloid mixed lineage leukemia, but negative in 6 cases of chronic myelogeneous leukemia (CML), significantly lower than that in AML (39/55, P<0.01) patients. There was no difference between the positive rates of chronic lymphocytic leukemia (CLL, 3/5) and B lineage ALL (9/28, P = 0.2389). The antibody was not found to recognize the differentiation stages of either ALL and AML subtypes.<br />Conclusion: 2D3 monoclonal antibody primarily recognizes AML cases. Further studies on cloning of gene encoding the antigen protein and its biological functions are warranted.
- Subjects :
- Adolescent
Adult
Aged
Antigens, Neoplasm immunology
Child
Child, Preschool
Female
Humans
Immunophenotyping
Infant
Leukemia, Lymphocytic, Chronic, B-Cell immunology
Leukocyte Common Antigens immunology
Male
Middle Aged
Antibodies, Monoclonal immunology
Antibodies, Neoplasm immunology
Leukemia, Myeloid, Acute immunology
Precursor Cell Lymphoblastic Leukemia-Lymphoma immunology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1008-9292
- Volume :
- 35
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 16924702
- Full Text :
- https://doi.org/10.3785/j.issn.1008-9292.2006.04.008